苜蓿转化细胞系核酸和蛋白质合成的研究     

谢经良  唐学玺 《中国海洋大学学报(自然科学版)》2003,33(2):249-252

以苜蓿转化细胞系和对照细胞系为材料 ,利用同位素标志方法研究了 3种大分子物质——DNA、RNA和蛋白质的合成动态。结果表明 ,与对照细胞系相比 ,转化细胞系的生长速度明显加快 ,DNA、RNA和蛋白质的合成旺盛 ,合成速度显著提高。同时 ,转化细胞系 DNA、RNA和蛋白质出现最大合成速度的时间均相应地延迟。  相似文献   

黄河三角洲潮滩剖面特征   总被引：4，自引：0，他引：4  

周良勇  李广雪  刘健  李安龙  邓声贵  温国义  赵东波 《海洋地质与第四纪地质》2006,26(2):1-8

利用验潮、潮滩水准测量、沉积物分析和遥感资料,分析了黄河三角洲不同岸段潮滩的特征。水准测量表明,侵蚀潮滩剖面形态为下凹,而淤积潮滩则上凸,由高潮线至低潮线,潮滩沉积物均变粗,这种变粗趋势在北部侵蚀岸段和河口侧缘明显,在河口南侧稳定海岸则不明显。潮滩沉积物含水量为12.2%~32.2%,平均为21%,海滩表层沉积物不排水剪切强度在0.1~0.35 kg/cm2之间。受局限岸滩的平面形态和剖面形态受到周围大坝地形影响,具有和砂质海滩类似的形态,显示波浪对该潮滩的作用强烈,根据剖面测量结果与遥感图像,发现河口北侧有一个小的冲积扇。  相似文献   

0220号热带风暴(米克拉)特征分析     

李孟植 《海洋预报》2003,20(3):7-11

南海是热带气旋活动较为频繁的地区之一。该地区因其独特的地理环境和复杂的热带大气环流系统，在热带气旋预报工作上存在一定的困难。本文在分析一些气象图表资料的基础上，对2002年第20号热带风暴(米克拉)的形成、发展及消亡进行了探讨和研究。主要从形成的背景环流，气旋的结构等方面入手，总结出一些该气旋所具备韵特有流场，从而为以后的南海自生热带气旋预报工作积累一定经验。  相似文献   

海底浅层介质切变波的初步研究   总被引：2，自引：0，他引：2  

卢博  李赶先  黄韶健 《热带海洋学报》2004,23(4):11-18

初步分析了中国沿海海底浅层介质切变波的传播特性,讨论了多种海底浅层介质切变波与压缩波、物理力学性质之间的关系,并与Hamilton和Chen等人的结果进行了比较。认为海底浅层介质存在切变模量,切变波可以被测量出来,切变波速为50—600m·s-1,测量频率在50—200kHz之间,用波速比可以评价海底浅层介质的应力 应变性质。研究工作有助于描述和评价中国沿海海底浅层介质声学物理性状,并为最终建立中国沿海海底地声模型提供依据。  相似文献   

利用牙鲆鳃细胞系分离和培养淋巴囊肿病毒   总被引：1，自引：4，他引：1  

吕宏旭  汪岷  李红岩  郭华荣  张士璀  包振民  周丽 《中国海洋大学学报(自然科学版)》2003,33(2):233-239

本文利用牙鲆鳃细胞系进行了养殖牙鲆淋巴囊肿病毒的分离及培养 ,并通过电镜对培养细胞中淋巴囊肿病毒的形态及感染循环进行了初步研究。将病鱼的淋巴囊肿组织无菌滤液接种牙鲆细胞系 ,细胞出现了明显的细胞病变 ( Cytopathic effect,CPE)。电镜观察在培养细胞的胞质中有病毒的包涵体 ,胞质中散在 6角形、5角形或圆形的病毒粒子 ,大小为 10 0～ 140 nm之间。在感染细胞的线粒体中也存在大量的病毒颗粒。  相似文献   

Effects of organochlorine compounds on cytochrome P450 aromatase activity in an immortal sea turtle cell line     

Keller JM  McClellan-Green P 《Marine environmental research》2004,58(2-5):347-351

Many classes of environmental contaminants affect the reproductive function of animals through interactions with the endocrine system. The primary components affected by endocrine active compounds (EACs) are the steroid receptors and the enzymes responsible for steroidogenesis. This study sought to develop an in vitro model for assessing EAC effects in sea turtles by examining their ability to alter cytochrome P450 aromatase (CYP19) activity. Aromatase is the enzyme responsible for the conversion of testosterone to estradiol. This enzyme is critical in the sexual differentiation of reptiles which demonstrate temperature-dependent sex determination. An immortal testis cell line GST-TS from a green sea turtle was grown in culture at 30 degrees C in RPMI 1640 media. The cells were exposed to three known aromatase inducers; dexamethasone (Dex), 8Br-cyclic AMP, or human chronic gonadotropin (HCG) and one aromatase inhibitor 4-androstenol-dione (4-OHA). In addition, the GST-TS cells were exposed to 0.1-30 microM atrazine and 3-100 microM 4,4'-DDE. The inducing compounds that have been shown to increase aromatase activity in other systems failed to induce aromatase activity in the GST-TS cells, yet exposure to the inhibiting compound, 4-OHA, did result in a significant reduction. Atrazine (0.1, 1.0 and 10 microM) significantly induced aromatase activity following a 24 h exposure, and 4,4'-DDE inhibited the activity but only at cytotoxic concentrations (100 microM). Based on these results, this in vitro model can be useful in examining the endocrine effects of EACs in sea turtles.  相似文献   

南黄海表层沉积物中氮的区域地球化学特征   总被引：5，自引：0，他引：5  

吕晓霞  宋金明 《海洋科学进展》2003,21(2):174-180

通过分级浸取分离的方法获得了南黄海表层沉积物不同形态的氮，首次探讨其分布的区域性特征：南黄海表层沉积物中各形态氮在不同测线上均呈现出波状分布。B测线的基本上呈现出中部海域高，两边海域低，而西部海域又略高于东部海域的趋势，D和E测线的基本上呈现出西高、东低的趋势，C测线的则呈现出西低、东高的趋势，F测线的变化趋势比较平缓，从整体上看，SOEF—N和TN的分布呈现出西部海区比东部海区低的趋势。受各测线上沉积物种类差异的影响，自北向南各形态氮的变化幅度逐渐减小，其中SOEF-N在各测线上的变化幅度最大，且自北向南降幅最大。受水动力及沉积物粒度的影响，不同形态氮在黄海槽内各站位沉积物中的含量较高。沉积物中各形态氮在不同测线上的分布还表明，陆源输入对南黄海沉积物中氮测线分布有较小的影响，海洋自生氮对南黄海表层沉积物中氮的形态、含量与分布具有重要影响。  相似文献   

使用GAMIT进行高精度基线向量解算的方法与实践   总被引：2，自引：0，他引：2  

徐杰  任超  孟黎 《海洋测绘》2007,27(6):29-32

GAM IT是国际上常用的GPS定位定轨软件,但由于其安装的平台(UN IX/LINUX)与我们通用的W indow s平台有所差异,使得GAM IT的入门较难。因此本文结合具体实例,介绍GAM IT的安装、基本操作以及应用GAM IT进行高精度基线向量解算,供初学者参考。  相似文献   

The Structure of A Turbulent Jet in A Crossflow-Effect of Jet-Crossflow Velocity     

李行伟  匡翠萍  陈国谦 《中国海洋工程》2002,(1)

A comprehensive numerical study on the three-dimensional structure of a turbulent jet in crossflow is performed. The jet-to-crossflow velocity ratio (R) varies in the range of 2 - 16; both vertical jets and inclined jets without excess streamwise momentum are considered. The numerical results of the Standard two-equation k-ε model show that the turbulent structure can be broadly categorised according to the jet-to-crossflow velocity ratio. For strong to moderate jet discharges, i.e. R> 4, the jet is characterized by a longitudinal transition through a bent-over phase during which the jet becomes almost parallel with the main freestream, to a sectional vortex-pair flow with double concentration maxima; the computed flow details and scalar mixing characteristics can be described by self-similar relations beyond a dimensionless distance of around 20-60. The similarity coefficients are only weakly dependent on R. The cross-section scalar field is kidney-shaped and bifurcated, vvith distinct double concentr  相似文献   

Ecology of beach wrack in northern New England with special reference to Orchestia platensis     

Manaf I. Behbehani  Robert A. Croker   《Estuarine, Coastal and Shelf Science》1982,15(6)

The northern New England beach wrack community with special reference to the cosmopolitan amphipod crustacean, Orchestia platensis, was examined at estuarine and open coastal habitats. Beach wrack was dominated by the plant genera Ascophyllum, Zostera, Spartina and Chondrus, and was most abundant during spring and late summer. Animal community numbers, biomass and frequency in fresh to moderately decomposed wrack were dominated by O. platensis throughout the year at all habitats; oligochaetes and Collembola were also important. The abundance of O. platensis showed high spatial and temporal variability, with low abundance generally associated with decreased amounts of wrack during colder months. An exception was the winter presence of the species at one estuarine habitat, in patchy aggregations within gravel-cobble refuges. The abundance of O. platensis averaged 1280 (0.04 m²)⁻¹, with a maximum of 7040 (0.04 m²)⁻¹. The life cycle of O. platensis is bivoltine, with summer-hatched young reaching maturity within 1 month. Laboratory studies indicate females with up to 4 broods (30 days)⁻¹, averaging 18 eggs brood⁻¹.Orchestia platensis is omnivorous, eating fresh plant tissue, live oligochaetes, Limulus eggs and diatom ‘fuzz’. The rate of laboratory consumption of algae and Zostera was 0.05 mg plant mg⁻¹ wet body weight day⁻¹. Presumptive predators of O. platensis are juvenile green crab, Carcinus maenus, and the earwig. Anisolabis maritima. The mobility, aggregation and aggressiveness of O. platensis assist the species in establishing and maintaining populations in the rigorous wrack habitat. The general competitive superiority of O. platensis over its congener, O. gammarella, and the co-occurrence of these species on both eastern and western Atlantic shores is discussed.  相似文献